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manufacturer of rpi kanamycin monosulfatekanamycin

  • Kanamycin Sulfate at Thomas Scientific

    Luria Broth, High Salt Formula, Powder, 1 KG. Growth Media > Powder. Substance Name: Miller's LB Broth. CAS Number: 91079-40-2, …

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  • Home - RPI

    Kanamycin sulfate from Streptomyces kanamyceticus. Synonyms: Kanamycin sulfate salt, Kanamycin A. CAS Number: 25389-94-0. Molecular Weight: 582.58. EC Number: 246-933-9.

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  • Kanamycin | Sigma-Aldrich

    PhytoTech Labs: Your global supplier and manufacturer of plant tissue culture production and research chemicals, media, and lab equipment. Our talented sales, customer service, and technical service teams …

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  • PhytoTech Labs

    were purchased from RPI. Antibiotics were used at the concentrations indicated: ampicillin (Amp), 100 g/ml; kanamycin (Kan), 50 g/ml; tetracycline, 10 g/ml. Purification of IntN1. The intN1 gene was cloned into pET28a as described previously (5) to construct pET28a-intN1 (see Table S1 in the supplemental material). The …

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  • Interactions of NBU1 IntN1 and Orf2x Proteins with ...

    2017-7-17 · The expression of the Rpi-vnt1.1 gene was analyzed using primers RT-Rpi-vnt1.1-S: 5′-GGT AAG GTA TTG GCT CTG-3′, RT-Rpi-vnt1.1-AS: 5′-CTT CTC AGC AAT CCA CAT A-3 ’ designed using AlleleID 7 (Premier Biosoft), producing an amplicon of 132 bp and normalized to the elongation factor ef1α (Nicot et al. 2005).

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  • R Avr gene expression study of Rpi-vnt1.1

    We Believe You Are Important, How Can We Help? Log In or Create a Profile. We Believe You Are Important, How Can We Help? 833.544.SHIP (7447)

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  • Chemicals Antibiotics, Antimycotics & Preservative

    3.1. Rpi-blb2-mediated HR to P. infestans AVRblb2 requires SGT, but not RAR1 or HSP90 Previously, we demonstrated that AVRblb2 is a modular protein with effector activity that triggers an Rpi-blb2-dependent HR in N. benthamiana [6]. However, little is known about the resistance pathways mediated by Rpi-blb2 [7]. Some proteins, …

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  • Rpi-blb2-mediated late blight resistance in Nicotiana ...

    As a first step in PCR amplification, DNA was denatured for 5 min at 94°C and finalised by an extra 5-min elongation step at 72°C. The amplification reactions were performed in a Biometra®T-Gradient or Biometra®Uno-II thermocycler (Westburg, Leusden, the Netherlands).

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  • An ancient R gene from the wild potato species

    R/Avr gene expression study of Rpi-vnt1.1 transgenic potato resistant to the Phytophthora infestans clonal lineage EC-1. Plant Cell, Tissue and Organ Culture (PCTOC), Jul 2017

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  • Kanamycin at Thomas Scientific

    We Believe You Are Important, How Can We Help? Log In or Create a Profile. We Believe You Are Important, How Can We Help? 833.544.SHIP (7447)

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  • Kanamycin Sulfate at Thomas Scientific

    were purchased from RPI. Antibiotics were used at the concentrations indicated: ampicillin (Amp), 100 g/ml; kanamycin (Kan), 50 g/ml; tetracycline, 10 g/ml. Purification of IntN1. The intN1 gene was cloned into pET28a as described previously (5) to construct pET28a-intN1 (see Table S1 in the supplemental material). The …

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  • Kanamycin A - thomassci.com

    2019-5-25 · selected for overnight growth (37 °C) in 10 mL of LB containing kanamycin (50 μg/mL). 6 x 1 L of TB (2 L Erlenmeyer flasks) supplemented with kanamycin (50 μg/mL), thiamine (1 mM), and glycerol (4% v/v) were inoculated with the 10 mL overnight seed cultures and incubated at 37 °C (200 rpm).

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  • Interactions of NBU1 IntN1 and Orf2x Proteins with ...

    2014-9-1 · Potato Rpi-blb2 encodes a protein with a coiled-coil-nucleotide binding site and leucine-rich repeat (CC-NBS-LRR) motif that recognizes the Phytophthora infestans AVRblb2 effector and triggers hypersensitive cell death (HCD). To better understand the components required for Rpi-blb2-mediated HCD in plants, we used virus-induced gene silencing to repress candidate genes in Rpi …

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  • Biochemical and Structural Characterization of MycCI,

    2021-1-3 · The potato late blight resistance gene Rpi-amr3 (Resistance to Phytophthora infestans) was identified in S. americanum, a possible diploid ancestor of S. nigrum (Ganapathi & Rao, 1987; Witek et al., 2016). Identification of a novel avirulence protein is an important step to develop disease resistance.

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  • Rpi-blb2-Mediated Hypersensitive Cell Death

    Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease for potato cultivation.Here, we describe the positional cloning of the Rpi-blb1 gene from the wild potato species Solanum bulbocastanum known for its high levels of resistance to late blight. The Rpi-blb1 locus, which confers full …

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  • Identification of RipAZ1 as an avirulence

    2019-12-19 · Constructing BBa_K1894001 shuttle vector. 1. Add overlaps to fragments 1, 2 and 3 using PCR according to manufacturer protocol 8. 2. Assemble fragments 1, 2 and 3 using Gibson assembly according to manufacturer protocol. Incubate for 1.5 hours. 3. LPerform PCR purification according to manufacturer …

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  • An ancient R gene from the wild potato species

    2021-6-30 · Hambarde et al. reveal EXO5 structures and its heretofore missed structure-specific nuclease activity for replication fork restart orchestrated with ATR kinase and BLM helicase. EXO5 depletion rescues FANCA-deficient cell survival and fork protection defects. High EXO5 expression predicts high mutation loads and …

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  • Neomycin Sulfate at Thomas Scientific

    2017-10-18 · with 50mgmL!1 of kanamycin. Primers from IDT were synthesized to perform site-directed mutagenesis on amino acid number 236 of the pET28a(þ)-tA16ACII construct, converting an isoleucine codon (ATT) to a threonine codon (ACC). The QuikChange Site-Directed Mutagenesis Kit from Agilent was used according to the manufacturer…

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  • Cloning and Expression of ... - www-heparin.rpi.edu

    Ribose-5-phosphate isomerase (Rpi) (EC 5.3.1.6) is an aldose-ketose isomerase that catalyses the inter-conversion between ... um containing 50 μg/ml of kanamycin to an initial OD 600 nm of 0.05., and growth . 102 Chapter 4 at 37 oC was continued to an ... NTA column according to the manufacturer’s instructions, and the column was …

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  • Rpi - blb2 gene from Solanum bulbocastanum

    We Believe You Are Important, How Can We Help? Log In or Create a Profile. We Believe You Are Important, How Can We Help? 833.544.SHIP (7447)

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  • University of Groningen Mutational impact of classical ...

    2021-1-3 · The potato late blight resistance gene Rpi-amr3 (Resistance to Phytophthora infestans) was identified in S. americanum, a possible diploid ancestor of S. nigrum (Ganapathi & Rao, 1987; Witek et al., 2016). Identification of a novel avirulence protein is an important step to develop disease resistance.

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  • Chemicals Antibiotics, Antimycotics & Preservative

    2014-6-23 · kanamycin (50 μg/ml), and streptomycin (50 μg/ml), or combinations of these antibiotics, were used when nec-essary. Cinnamic acid, p-coumaric acid, and caffeic acid were purchased from MP Biomedicals Inc. (Irvine, CA). Naringenin was purchased from Sigma (St. Louis, MO). Ferulic acid, eriodictyol, apigenin, luteolin, …

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  • Identification of RipAZ1 as an avirulence

    2014-2-27 · The Rpi-blb2 recognizes the presence of the Phytophthora infestans AVRblb2 and initiates effector-triggered immunity (ETI). We performed gain-of-function and loss-of-function studies in Nicotiana benthamiana to elucidate Rpi-blb2-mediated resistance to P. infestans.Rpi-blb2 triggered a hypersensitive response …

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  • Effendi Leonard . Joseph Chemler . Kok Hong Lim .

    2020-4-30 · Both cell types were cultured in Luria Broth (LB) media (RPI) with 100 μg/mL ampicillin (Fisher Scientific) added for selective growth. Each 1-L LB + Amp culture was inoculated with 10 mL overnights and grown at 37°C with shaking until reaching an OD 600 of 0.6–0.8. Subsequent induction with 0.2 mM isopropyl β-D …

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  • Rpi‐blb2‐mediated late blight resistance in

    2016-6-23 · In Staphylococcus aureus, metabolism is intimately linked with virulence determinant biosynthesis, and several metabolite-responsive regulators have been reported to mediate this linkage.S. aureus possesses at least three members of the RpiR family of transcriptional regulators. Of the three RpiR homologs, RpiRc is a …

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  • University of Groningen Mutational impact of classical ...

    Ribose-5-phosphate isomerase (Rpi) (EC 5.3.1.6) is an aldose-ketose isomerase that catalyses the inter-conversion between ... um containing 50 μg/ml of kanamycin to an initial OD 600 nm of 0.05., and growth . 102 Chapter 4 at 37 oC was continued to an ... NTA column according to the manufacturer’s instructions, and the column was …

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  • Addgene: Plasmid Cloning by PCR (with Protocols)

    2004-11-1 · RPI-10 is predicted to contain a bipartite nuclear localization sequence , and may serve to communicate signals between the cytoplasm and the nucleus. RPI-11 showed similarity to a protein of unknown function, and was an especially strong RPS2-interactor. The remaining RPIs that we identified also merit brief discussion.

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  • Identification and functional analysis of

    2017-12-15 · kanamycin. After five cultivations, the culture was streaked on LB agar with kanamycin. Separate colonies were inoculated on LB agar with 30 mg 1litergen op Zoom, 4612 PL, The Netherlands. gentamicin. Colonies that had lost their Gmr phenotype were considered to be cured of the pNW1C plasmid.

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  • Postnatal Expression of Neurotrophic Factors

    Multiple recognition of RXLR effectors is associated with nonhost resistance of pepper against Phytophthora infestans Hyun-Ah Lee1,2*, Shin-Young Kim1,2*, Sang-Keun Oh1, Seon-In Yeom1,2, Saet-Byul Kim1,2, Myung-Shin Kim1,2, Sophien Kamoun3 and Doil Choi1,2 1Department of Plant Science, College of Agriculture …

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  • Transcriptome Analysis of a Phenol-Producing

    2014-8-29 · Fire blight is caused by Erwinia amylovora and is the most destructive bacterial disease of apples and pears worldwide. In this study, we found that E. amylovora argD(1000)::Tn5, an argD Tn5 transposon mutant that has the Tn5 transposon inserted after nucleotide 999 in the argD gene-coding region, was an …

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  • Multiple recognition of RXLR effectors is associated

    The most significant threat to pepper production worldwide is the Phytophthora blight, which is caused by the oomycete pathogen, Phytophthora capsici Leonian. In an effort to help control this disease, we isolated and characterized a P. capsici resistance gene, CaRGA2, from a high resistant pepper (C. annuum CM334) and …

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  • Mutation of the Erwinia amylovora argD gene

    Evaluation of transgenic King Edward and B101 lines transformed with the triple resistance (3R: RB, Rpi-blb2, and Rpi-vnt1.1) gene stack against P. infestans in detached-leaf assays. (A) Detached-leaf assay results for wild-type King Edward (Control) and the 3R-transformed King Edward lines (3R-K1 to K5) at 7 dpi.

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  • IJMS | Free Full-Text | Characteristic of the

    2005-7-1 · Ethambutol (EMB) is in use worldwide as a first-line anti-tuberculosis drug and substitutions in codon 306 of the embB gene are the most common mutations found in EMB resistant Mycobacterium tuberculosis (MTB) strains. Pyrosequencing is a real time sequencing method able to rapidly detect mutations in a large number …

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  • FAQ 1: How much antibiotic should I use for

    2019-1-15 · Kanamycin: 30 : 50 : Chloramphenicol: 10 : 20 : Tetracycline: 12.5 : 25 : Spectinomycin: 30-100* 100 : Hygromycin: 200 : 200 # Blasticidin: not determined : 50 # We originally used 50µg/ml hygromycin but found that it gave a high background. We now use 200µg/ml. Hygromycin should be filter-sterilized before addition as the manufacturer …

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  • Chemoenzymatic total synthesis and structural ...

    2019-5-25 · from BioExpress (VWR). Kanamycin sulfate, isopropyl-β-D-thiogalactopyranoside (IPTG), and dithiothreitol (DTT) were obtained from Gold Biotechnology. Tris-HCl, ampicillin disodium salt, and NADP+ were purchased from Amresco. Chloramphenicol was obtained from Roche. Thiamine was purchased from Chem-Impex. Lysozyme was purchased from RPI.

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  • Rpi-blb2-mediated late blight resistance in

    2014-4-2 · The Rpi-blb2 recognizes the presence of the Phytophthora infestans AVRblb2 and initiates effector-triggered immunity (ETI). We performed gain-of-function and loss-of-function studies in Nicotiana benthamiana to elucidate Rpi-blb2-mediated resistance to P. infestans.Rpi-blb2 triggered a hypersensitive response …

    Get Price
  • Identification and functional analysis of

    2004-11-1 · RPI-10 is predicted to contain a bipartite nuclear localization sequence , and may serve to communicate signals between the cytoplasm and the nucleus. RPI-11 showed similarity to a protein of unknown function, and was an especially strong RPS2-interactor. The remaining RPIs that we identified also merit brief discussion.

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  • Construction and functional characterization of

    2017-9-13 · ORIGINAL ARTICLE Construction and functional characterization of truncated versions of recombinant keratanase II from Bacillus circulans HaishengWang1,2 & Wenqin He 2 & PeixiaJiang3,4 & Yanlei Yu 3 & LeiLin3 & XiaojunSun3 & Mattheos Koffas2 & Fuming Zhang2 & Robert J. Linhardt2,3,5 …

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  • In Planta Expression Screens of Phytophthora

    In the Rpi-blb1 coinfiltrations, the HR was observed with the two PexRD6/IpiO clones starting at 4 DAI, and for Rpi-blb2, the HR was observed with both PexRD39 and PexRD40 constructs starting at 3 DAI (Figures 5B to 5D). Altogether, these experiments indicate that the identified clones are specifically recognized by the …

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  • Metabolic engineering of Yarrowia lipolytica for ...

    2020-11-16 · Functional sugar alcohols have been widely used in the food, medicine, and pharmaceutical industries for their unique properties. Among these, erythritol is a zero calories sweetener produced by the yeast Yarrowia lipolytica. However, in wild-type strains, erythritol is produced with low productivity and yield and only under …

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  • Gene conferring resistance to Phytophthera

    DATABASE EMBL ÄOnlineÜ EMBL; 5 September 2001 (2001-09-05) PAN Q. ET AL.: 'Lycopersicon esculentum isolate Q194 nucleotide binding region of resistance-like gene, partial sequence' Database accession no. AF404480 XP002206417 -& PAN Q. ET AL.: 'Comparative genetics of nucleotide binding site-leucin rich repeat …

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  • FUNCTIONAL R-GENE FROM SOLANUM

    2010-6-24 · The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. bulbocastanum.More over, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycte …

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  • Structural Basis for Substrate Recognition by the

    Verardi et al. reveal the structural basis of interaction between DHHC17, a neuronal palmitoyltransferase, and SNAP25b, a canonical substrate. They show the role of critical residues for substrate binding and palmitate transfer using in vitro and cell-based assays and show the involvement of the same residues in binding …

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  • Grainger SDS Lookup

    product name: kanamycin monosulfate [kanamycin a] common name: kanamycin a rpi catalog #: k22000 supplier's name: research products international corp. suppliers address: 410 n business center drive mount prospect, il 60056 emergency contact: 1-800-424-9300 other information: 847-635-7330

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  • Lassomycin, a Ribosomally Synthesized Cyclic

    2014-4-24 · tuberculosis mc 2 6020 pBEN mCherry used to inoculate (0.5%–1% inoculum) 50 ml of 7H9 with OADC, glycerol, casamino acids, taloxapol, panthothenic acid, lysin, and 50 μg/ml of kanamycin. The culture was incubated at 37°C with shaking until the optical density 600 reached 0.4–0.5.

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  • FAQ 1: How much antibiotic should I use for

    2019-1-15 · Kanamycin: 30 : 50 : Chloramphenicol: 10 : 20 : Tetracycline: 12.5 : 25 : Spectinomycin: 30-100* 100 : Hygromycin: 200 : 200 # Blasticidin: not determined : 50 # We originally used 50µg/ml hygromycin but found that it gave a high background. We now use 200µg/ml. Hygromycin should be filter-sterilized before addition as the manufacturer …

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  • Enzyme and Microbial Technology

    2017-5-1 · on LB agarplates with 50 g/mL kanamycin. The colonies were replicated by stamping on velvet with a subsequent transfer on a new plate. After growth of colonies, screening top-agarose (50mM sodium acetate, 2.5mM calcium chloride x 2 H2O, 170mM NaCl, 2.5mM esculin hydrate, 0.4mM ammonium ferric citrat, 1.2% …

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  • Frontiers | Conserved RXLR Effector Genes of

    2009-9-1 · The Irish potato famine pathogen Phytophthora infestans is predicted to secrete hundreds of effector proteins. To address the challenge of assigning biological functions to computationally predicted effector genes, we combined allele mining with high-throughput in planta expression. We developed a library of 62 …

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  • In Planta Expression Screens of Phytophthora

    2018-8-11 · RNA was isolated following manufacturer's instructions with the use of GeneMATRIX Universal RNA Purification Kit (EURx, Poland) from 10 ml of L. lactis IL1403 and L. lactis IL1403ΔglaR cultures grown in G-M17, C-M17, Gal-M17 or GalC-M17 and collected from midexponential phase (OD 600 = 0.6). RNA was isolated …

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  • GlaR (YugA)—a novel RpiR‐family transcription

    2020-3-11 · To study xenosiderophore-mediated iron uptake in a more controlled setting, we depleted trace elements from media using a polyvalent ion exchange resin, added back trace elements except iron, and assessed growth of B. thetaiotaomicron (Figure S2A). The apo form of both catecholate siderophores …

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  • Xenosiderophore Utilization Promotes

    DATABASE EMBL ÄOnlineÜ EMBL; 5 September 2001 (2001-09-05) PAN Q. ET AL.: 'Lycopersicon esculentum isolate Q194 nucleotide binding region of resistance-like gene, partial sequence' Database accession no. AF404480 XP002206417 -& PAN Q. ET AL.: 'Comparative genetics of nucleotide binding site-leucin rich repeat …

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  • Gene conferring resistance to Phytophthera

    2010-6-24 · The invention relates to a resistance gene and functional homologues or fragments thereof isolated from S. bulbocastanum.More over, the invention relates to the use of said resistance gene, for example the use of said resistance gene in a method to increase or confer at least partial resistance in a plant to an oomycte …

    Get Price
  • FAQ 1: How much antibiotic should I use for

    2019-1-15 · Kanamycin: 30 : 50 : Chloramphenicol: 10 : 20 : Tetracycline: 12.5 : 25 : Spectinomycin: 30-100* 100 : Hygromycin: 200 : 200 # Blasticidin: not determined : 50 # We originally used 50µg/ml hygromycin but found that it gave a high background. We now use 200µg/ml. Hygromycin should be filter-sterilized before addition as the manufacturer …

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  • Stacking three late blight resistance genes from

    2018-11-22 · The selectable marker gene for vector selection in bacteria is the aminoglycoside phosphotransferase gene conferring resistance to kanamycin. The Rpi-blb2 gene was chemically synthesized by Entelechon GmbH (Germany) using nucleotides 1–5730 of the 7967 bp genomic fragment bearing the full Rpi-blb2 …

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  • Enzyme and Microbial Technology

    2017-5-1 · on LB agarplates with 50 g/mL kanamycin. The colonies were replicated by stamping on velvet with a subsequent transfer on a new plate. After growth of colonies, screening top-agarose (50mM sodium acetate, 2.5mM calcium chloride x 2 H2O, 170mM NaCl, 2.5mM esculin hydrate, 0.4mM ammonium ferric citrat, 1.2% …

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  • RPE, a plant gene involved in early developmental

    1998-12-1 · Of the 435 T2 seeds tested in bulk, 287 grew on kanamycin medium and displayed a normal phenotype compared with the wild type. This frequency fitted well with the hypothesis of the ratio of kanamycin-resistant (K r) to sensitive (K s) being 2:1 (χ 2 = 0.09; P >0.05), suggesting also that the T-DNA was inserted in one …

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  • The kgmB gene, encoding ribosomal RNA

    2004-9-30 · The KgmB methylase (the kanamycin–gentamicin resistance methylase from Streptomyces tenebrarius) acts at G-1405 of 16S rRNA within the sequence CGUCA that is also found 6 bp in front of ribosomal binding site of the kgmB gene. The kgmB∷lacZ gene and operon fusions were used in order to test for …

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  • African Journal of Biotechnology - extreme

    The latter has three similar allelic variants; Rpi-vnt1.1, Rpi-vnt1.2 and Rpi-vnt1.3. These R genes confer extreme resistance to P. infestans but have not been extensively used in potato breeding due to the time lag to produce acceptable varieties and the short-lasting resistance mediated by single R genes …

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  • US Patent Application for CLONING AND

    Rpi-chc1 homologs were PCR amplified using the long range high fidelity thermostable DNA polymerase Phusion® according to the manufacturer's instructions (New England Biolabs). Primers were designed, overlapping the start and stop codons of Rpi-chc1 and contained AttB1 and AttB2 extensions (MN595 and …

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  • GlaR (YugA)—a novel RpiR‐family transcription

    2018-8-11 · RNA was isolated following manufacturer's instructions with the use of GeneMATRIX Universal RNA Purification Kit (EURx, Poland) from 10 ml of L. lactis IL1403 and L. lactis IL1403ΔglaR cultures grown in G-M17, C-M17, Gal-M17 or GalC-M17 and collected from midexponential phase (OD 600 = 0.6). RNA was isolated …

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  • Biomolecules | Free Full-Text | QueF-Like, a Non ...

    Sigma-Aldrich® Lab & Production materials that help you Unleash the Impossible. Explore our range of lab, diagnostic and bulk products, hear stories from inspiring scientists like you, and more.

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  • RPE, a plant gene involved in early developmental

    1998-12-1 · Of the 435 T2 seeds tested in bulk, 287 grew on kanamycin medium and displayed a normal phenotype compared with the wild type. This frequency fitted well with the hypothesis of the ratio of kanamycin-resistant (K r) to sensitive (K s) being 2:1 (χ 2 = 0.09; P >0.05), suggesting also that the T-DNA was inserted in one …

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  • An in vivo internal deletion in the N‐terminus

    2006-2-17 · Tobacco plants were transformed and selected on media containing 100 mg l −1 kanamycin as previously described (Horsch et al., 1985). Complementing the E. coli methionine auxotroph The three forms of cDNA encoding the full‐length and two deleted forms of CGS were amplified from a cDNA flower library using primers …

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  • PLOS ONE: High-Resolution Sequence-Function

    2015-3-19 · Comprehensive sequence-function mapping involves detailing the fitness contribution of every possible single mutation to a gene by comparing the abundance of each library variant before and after selection for the phenotype of interest. Deep sequencing of library DNA allows frequency reconstruction for tens …

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  • Generation and Use of Recombinant Galectins -

    2021-3-3 · Dissolve 2.5 g kanamycin sulfate from Streptomyces kanamyceticus (MilliporeSigma, K1377) in 50 ml sterilized water. Pass through a 0.22-µm filter and aliquot into 1.5-ml vials and store at −20°C for up to 6 months. LB broth. Dissolve 20 mg of LB broth powder (RPI Research Products International, L24060) in 900 ml H …

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  • Evaluation of the available cholesterol

    Cholesterol is an essential component of the mammalian plasma membrane involved in diverse cellular processes. Our recent quantitative imaging analysis using ratiometric cholesterol sensors showed that the available cholesterol concentration in the inner leaflet of the plasma membrane (IPM) is low in unstimulated cells and …

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  • Department of Microbiology, Box 357242, University

    ing to the manufacturer's protocol. DNA was introduced into A. tumefaciens by electroporation as described (22). Plasmid Constructions. The salient features of pKP506, pKP509, and pKP510 are diagrammed in Fig. 1. pSc4128, a gift from B. Brewer (University of Washington) (24) consists of a 7.7 kb Xho I-Sal I yeast DNA …

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  • Optimization of Protein Thermostability and

    2020-4-30 · This paper describes an approach for protein engineering to recognize new DNA targets. The authors demonstrate that counteracting the destabilizing effect of increasing numbers of mutations, by computationally “pre-stabilizing” the protein scaffold, improves the recovery of active engineered proteins and robust …

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  • Regulation of reactive oxygen species during plant

    2020-4-15 · Production of reactive oxygen species (ROS) is critical for successful activation of immune responses against pathogen infection. The plant NADPH oxidase RBOHD is a primary player in ROS ...

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  • Metabolic engineering of Mortierella alpina for ...

    2015-12-23 · Although some microorganisms can convert glycerol into valuable products such as polyunsaturated fatty acids, the yields are relative low due primarily to an inefficient assimilation of glycerol. Mortierella alpina is an oleaginous fungus which preferentially uses glucose over glycerol as the carbon source for fatty acid …

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  • Membrane association of monotopic

    2018-5-16 · A 20-g batch of cells was resuspended in 100 mL buffer A (50 mM HEPES, pH 7.5, 150 mM NaCl) containing 50 mg lysozyme (RPI, cat. no. L38100), 100 µL EDTA-free protease inhibitor cocktail (EMD cat ...

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  • A Phytophthora sojae CRN effector mediates

    2021-3-12 · Author summary CRN effectors are conserved in diverse pathogens of plants, animals, and insects, and highly expanded in Phytophthora species. Nevertheless, little is known about their functions, targets, and action mechanisms. Here, we characterized a kinase-domain-containing CRN effector (CRN78) in a …

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  • Towards a Rigorous Network of Protein-Protein

    2011-6-28 · During selection and culturing transformants, G418 (RPI corp., Mt. Prospect, IL) was added to a final concentration of 400 µg/ml. G418 was used in place of kanamycin because it was more effective for selection of the kanamycin resistance marker in JW801.

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  • JCI - The composition of the microbiota

    2016-6-20 · Mice in Abx treatment cages received a daily gavage with 200 μl of Abx based on a previous report containing gentamycin (0.35 mg/ml, Fresenius Kabi), kanamycin (5.25 mg/ml, Gibco, Thermo Fisher Scientific), colistin (8500 U, RPI), metronidazole (2.15 mg/ml, Sigma-Aldrich), and vancomycin (0.5 mg/ml, Hospira) …

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  • Generation and Use of Recombinant Galectins -

    2021-3-3 · Dissolve 2.5 g kanamycin sulfate from Streptomyces kanamyceticus (MilliporeSigma, K1377) in 50 ml sterilized water. Pass through a 0.22-µm filter and aliquot into 1.5-ml vials and store at −20°C for up to 6 months. LB broth. Dissolve 20 mg of LB broth powder (RPI Research Products International, L24060) in 900 ml H …

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  • A new mutant of Arabidopsis disturbed in its roots,

    horizontal rows, on agar medium containing kanamycin (50 mg nested oligonucleotides complementary to sequences of the T-DNA 1_I). Dishes were kept for 3 weeks inclined at 60° on the horizontal left-border (TL-1, TL-2, and TL-3) and one degenerate (DEG-2) plane to check for waving, slanting, and any deviation …

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  • Frontiers | Use of Agrobacterium rhizogenes

    2016-5-24 · The genetic transformation of monocot grasses is a resource intensive process, the quality and efficiency of which is dependent in part upon the method of DNA introduction, as well as the ability to effectively separate transformed from wildtype tissue. Agrobacterium-mediated transformation of Brachypodium has …

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  • Structure and Function of the Genomically

    2019-5-25 · The Gram-positive pathogen Staphylococcus aureus is a leading cause of global morbidity and mortality. Like many multi-drug-resistant organisms, S. aureus contains antibiotic-modifying enzymes that facilitate resistance to a multitude of antimicrobial compounds. FosB is a Mn2+-dependent fosfomycin-inactivating …

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  • Metabolic engineering of Mortierella alpina for ...

    2015-12-23 · Although some microorganisms can convert glycerol into valuable products such as polyunsaturated fatty acids, the yields are relative low due primarily to an inefficient assimilation of glycerol. Mortierella alpina is an oleaginous fungus which preferentially uses glucose over glycerol as the carbon source for fatty acid …

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  • Comparative secretome analysis of different smut

    2019-8-16 · Tilletia horrida is a basidiomycete fungus that causes rice kernel smut, one of the most important rice diseases in hybrid rice growing areas worldwide. However, little is known about its mechanisms of pathogenicity. We previously reported the genome of T. horrida, and 597 genes that encoded secreted proteins …

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  • Onl Er nph 12861 1. - Seoul National University

    2019-4-29 · genes are Rpi-blb1, which interacts with Avrblb1 (Vleeshouw-ers etal., 2008), and Rpi-blb2, which recognizes Avrblb2 (Oh etal., 2009). This genomic information and the development of experimental methods that enable effector-omics analyses of plant–microbe interactions, provide a promising approach for …

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  • Comparative secretome analysis of different smut

    2019-8-16 · Tilletia horrida is a basidiomycete fungus that causes rice kernel smut, one of the most important rice diseases in hybrid rice growing areas worldwide. However, little is known about its mechanisms of pathogenicity. We previously reported the genome of T. horrida, and 597 genes that encoded secreted proteins …

    Get Price
  • Identification and functional analysis of the NLP

    2018-3-23 · Phytophthora capsici is a hemibiotrophic, phytopathogenic oomycete that infects a wide range of crops, resulting in significant economic losses worldwide. By means of a diverse arsenal of secreted effector proteins, hemibiotrophic pathogens may manipulate plant cell death to establish a successful infection and …

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  • Manufacture of five-carbon sugars and sugar

    The invention is thus directed to a method of producing five-carbon sugars and sugar alcohols, especially xylitol, as well as other PPP intermediates or products derived from the same, by fermentation of six-carbon sugars (preferably glucose), in a genetically modified and engineered pathway in a single microbial host.

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  • Multiple recognition of RXLR effectors is

    2014-6-2 · Two of these genes are Rpi-blb1, which interacts with Avrblb1 (Vleeshouwers et al., 2008), and Rpi-blb2, which recognizes Avrblb2 (Oh et al., 2009). This genomic information and the development of experimental methods that enable effector-omics analyses of plant–microbe interactions, provide a promising …

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  • (PDF) In Planta Expression Screens of

    With the Avrblb genes at hand, we are now in a position to monitor the potential emergence of virulent races that may accompany the agricultural deployment of the Rpi-blb genes and rigorously assess the broad-spectrum activities reported for Rpi-blb1 and Rpi-blb2 (Helgeson et al., 1998;Song et al., 2003;van der Vossen et al., …

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  • Onl Er nph 12861 1. - Seoul National University

    2019-4-29 · genes are Rpi-blb1, which interacts with Avrblb1 (Vleeshouw-ers etal., 2008), and Rpi-blb2, which recognizes Avrblb2 (Oh etal., 2009). This genomic information and the development of experimental methods that enable effector-omics analyses of plant–microbe interactions, provide a promising approach for …

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  • Organization and Expression of the Polynucleotide

    Polynucleotide phosphorylase (PNPase) is a 3′-5′-exoribonuclease that catalyzes the phosphorolytic degradation of RNAs in Escherichia coli and other bacteria (14, 15, 35).PNPase is also a component of the RNA degradosome in E. coli (9, 40, 41).More recently, it has been shown that PNPase also functions as an alternative poly(A) …

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  • The Ph-3 gene from Solanum pimpinellifolium

    The Ph-3 gene is located at the end of the long arm of chromosome 9, a region carrying many Rpi genes in Solanaceae. In Solanum species, Rpi genes including Rpi-vnt1.1, Rpimcq1, R8, R9a, Rpi-edn2 and Rpi-dlc1, are located in this region (Pel et al. 2009; Smilde et al. 2005; Jo et al. 2011; Jo 2013; Verzaux 2010; Golas et al. 2010).

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  • Frontiers | A Candidate Secreted Effector Protein

    2020-11-24 · Powdery mildew infects a wide range of crops and economic plants, causing substantial losses. Rubber trees (Hevea brasiliensis) are the primary source of natural rubber, and powdery mildew infection causes significant losses to natural rubber yields. How the causal agent, Erysiphe quercicola, establishes successful …

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  • Structure determination protocol for

    The transmembrane domains of many type I/II membrane proteins oligomerize in the lipid bilayer, mediating protein assembly and clustering that are critical to their function. The authors provide a ...

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