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  • An outbreak of trimethoprim/sulfamethoxazole

    We were able to isolate S. maltophilia from the neuroendoscopy, but not from other environmental specimens in NICU or the operation room. A review of all medical records during one month before the first case indicated no other cases that were positive for S. maltophilia in the NICU, which we considered a possible source of the infection. Analysis of the records indicated that the neuroendoscopy was reprocessed using a glutaraldehyde disinfectant for sterilization…

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  • Pharmacokinetics of antibacterial agents in the CSF of ...

    Cefuroxime is the only exception and is usually avoided due to its adverse effects and slower sterilization of the CSF than third-generation agents. Ceftriaxone, cefotaxime, ceftazidime, cefixime and cefepime have been studied in children and are all able to adequately penetrate the CSF.

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  • Enrichment of endophytic Actinobacteria in roots and ...

    2020-1-24 · Roots and rhizomes were sliced into 1–2 cm pieces, weighed and surface sterilized with 2% NaClO for 20 min. Thereafter, the samples were rinsed three times in sterile water for 1 min, dried on sterilized filter paper, and ground in 5 ml of phosphate-buffered saline using sterile mortars and pestles.

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  • Sulfamethoxazole persistence in a river water

    2019-9-1 · Sulfamethoxazole (SMX) is a sulphonamide antibiotic commonly used in human and veterinary medicine due to its broad-spectrum action. It is prescribed to treat respiratory, urinary tract, skin and gastrointestinal infections and is also widely used in aquaculture and livestock breeding for animal health and in some countries as a growth promoter

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  • Isolation of Bacterial Endophytes from Phalaris ...

    2020-10-1 · The degraded products of SMX were extracted from the aqueous phase by ethyl acetate. The products were identified by using GC-MS (7890B System, 5977B MSD, Agilent Technologies, USA) according to the methods described by Kurade et al. (2019). The GC-MS was equipped with an HP-5MS capillary column (30 m × 250 mm, 0.25 mm) (Agilent J&W, USA) and a mass spectrometer.

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  • Transcriptomics of Planococcus kocurii O516 reveals

    2017-8-28 · Currently, methods of sterilization described either in- vivo or in-vitro, include autoclaving [4,5], pasteurization [6-8], extracorporeal irradiation [9-12], microwave [13],

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  • Which is the best method of sterilization for recycled ...

    2014-9-26 · Bacteria colonies on MH culture plate were picked up into 10 μl sterilization water, after bacteria degeneration, heated it at 99 °C for 10 min, and then took the supernatant as template by centrifugation processing. TaKaRa 16S rDNA bacterial identification PCR kit (Code No. D310, TaKaRa, Dalian, China) was used for PCR amplification.

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  • A case report of intracranial infection caused by ...

    2016-12-18 · abscesses fluids and were cultivated on Lowenstein-Jensen solid medium for up to four weeks at 37°C.15 A detailed his-tory of patients was obtained by the Public Health Surveil-lance System. We were not able to inspect environmental conditions such as sterilization …

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  • Molecular identification and typing of Mycobacterium ...

    2021-1-21 · vaporization. Adsorption and biodegradation were found to be the main routes for OMPs removal for all target OMPs. Target OMPs responded to the two dominant removal routes in different ways: (1) complete adsorption, (2) strong biodegradation and weak adsorption, (3) medium biodegradation and adsorption, and (4) weak sorption and weak

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  • Pharmacokinetics of antibacterial agents in the CSF of ...

    Cephalosporins vary greatly in regard to their CSF penetration. Few first- and second-generation cephalosporins are able to reach higher CSF concentrations. Cefuroxime is the only exception and is usually avoided due to its adverse effects and slower sterilization of the CSF than third-generation agents.

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  • An outbreak of trimethoprim/sulfamethoxazole

    We were able to isolate S. maltophilia from the neuroendoscopy, but not from other environmental specimens in NICU or the operation room. A review of all medical records during one month before the first case indicated no other cases that were positive for S. maltophilia in the NICU, which we considered a possible source of the infection ...

    Get Price
  • Effects of ultraviolet disinfection on antibiotic ...

    2017-4-29 · Water samples were collected twice a month (between March and June 2013) from influents and secondary effluents of a municipal WWTP in Xi'an, China. The biological treatment of the WWTP is an anoxic/anaerobic/oxic process. All samples were collected using 500-ml sterile containers, stored at 4°C and analysed within 24 h.

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  • Pharmacokinetics of sulfadimethoxine and

    The pharmacokinetics were studied of sulfadimethoxine (SDM) or sulfamethoxazole (SMX) in combination with trimethoprim (TMP) administered as a single oral dose (25 mg + 5 mg per kg body weight) to two groups of 6 healthy pigs. The elimination

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  • Treatment strategies for persistent methicillin-resistant

    able data and virtually no head-to-head trials. Suggested treatment includes high-dose daptomycin (10 mg/kg/d) in combination with gentamicin, rifampin, linezolid, trimethoprim- sulfamethoxazole (TMP-SMX) or a β-lactam antibiotic.4 There are no recommended salvage combinations with vancomycin. Furthermore, although

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  • Isolation of Bacterial Endophytes from Phalaris ...

    (2mg/l, both). All plates were prepared in triplicate and incubated at 25°C for 7 days. A 100μl sample of the third rinsing water was plated to confirm the effi-ciency of sterilization. The colonies on the plates were counted and the number of colony forming units per gram …

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  • Surgical Site Infections Caused by Rapidly Growing ...

    2020-5-22 · Rapidly growing nontuberculous mycobacteria (NTM) such as M. fortuitum and M. chelonae are ubiquitous; have been isolated from natural water, tap water, and water used in showers in hospitals; and can cause surgical site infections. A retrospective chart review at a tertiary care referral hospital was carried out. Patients with SSI in whom wound cultures grew rapidly growing NTM or ...

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  • Streptococcus pneumoniae Infections: Microbiology ...

    2021-4-29 · 1 If the surveillance overlapped 2 calendar years while covering 1 respiratory season, data were classified by the latter year. Adapted from: Stein CR, et al. Emerg Infect Dis 2003;9:211-216. Resistance to macrolides, tetracyclines, and trimethoprim-sulfamethoxazole is also widespread (Table 6).

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  • Can bactrim be used to treat chlamydia? -

    2020-3-10 · The clinical observations suggested that of the antibiotics studied, doxycycline, erythromycin, and trimethoprim-sulfamethoxazole were effective for treatment of chlamydial infection and nongonococcal urethritis. Untreated patients had symptoms …

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  • Bioneer Pacific - ExiPrep™ 16 Plus

    A.B.N. 19 133 059 355. My Favourites Register Log on. cart

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  • Which is the best method of sterilization for recycled ...

    2015-4-15 · The rabbits were able to weight bear immediately on both hind legs. The surgical procedures were performed by a single surgeon to achieve a uniform technique. After surgery, all rabbits received antibiotic (Kombitrim 240 or Sulfamethoxazole 200 mg/Trimethoprim 40 mg, 1 ml/10 kg IM) and analgesia (Meloxicam 5 mg/ml, 0.3 ml/kg IM) for five days.

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  • Antimicrobial Resistance Encountered in Garbage

    In the dumpsites and garbage collection areas that had high CFUs, such as Muthurwa estate dumpsite, Central police garbage collection area, City market garbage collection area, City park market dumpsite Figure 2(a), there were high resistance prevalence’s of above 25% to streptomycin, ampicillin, tetracycline and trimethoprim sulfamethoxazole ...

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  • Treatment strategies for persistent methicillin-resistant

    able data and virtually no head-to-head trials. Suggested treatment includes high-dose daptomycin (10 mg/kg/d) in combination with gentamicin, rifampin, linezolid, trimethoprim- sulfamethoxazole (TMP-SMX) or a β-lactam antibiotic.4 There are no recommended salvage combinations with vancomycin. Furthermore, although

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  • Endophytic Lifestyle of Global Clones of Extended

    isolates were able to endophytically colonize common bean ( Phaseolus vulgaris ) seed-lings, showed resistance to acid pH, and had a multidrug-resistant (MDR) pro file to clini-cally relevant antibiotics (i.e., broad-spectrum cephalosporins, aminoglycosides, and fluo-roquinolones).

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  • Streptococcus pneumoniae Infections: Microbiology ...

    2021-4-29 · 1 If the surveillance overlapped 2 calendar years while covering 1 respiratory season, data were classified by the latter year. Adapted from: Stein CR, et al. Emerg Infect Dis 2003;9:211-216. Resistance to macrolides, tetracyclines, and trimethoprim-sulfamethoxazole is also widespread (Table 6).

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  • MARUTO ML Film | MARUTO SANGYO CO., LTD.

    2021-4-15 · We were introduced to MARUTO ML Film, and found that we were able to raise our production speed while still maintaining sealing stability. Our shot rate increased from 100 per minute to 170 per minute, resulting in a major improvement in production efficiency. The 70% improvement of production speed is not necessarily the thing which can be ...

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  • A case report of intracranial infection caused by ...

    2014-9-26 · Shewanella putrefaciens is as yet reputed to be a rare conditional pathogen. In recent years, some clinical infections caused by Shewanella putrefaciens came into view, and it was possible for the bacteria to be isolated from blood, pus, urine, sputum, and wound secretions, etc. A transferred patient who suffered from intracranial infection after operation of cerebral hemorrhage was admitted ...

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  • Bioneer Pacific - ExiPrep™ 16 Plus

    A.B.N. 19 133 059 355. My Favourites Register Log on. cart

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  • 20.106J – Systems Microbiology Prof. Schauer

    2020-12-30 · 20.106J – Systems Microbiology Lecture 16 Prof. Schauer ¾ Chapter 20 Microbial growth control o Physical antimicrobial control o Chemicals that are used externally

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  • The Fate of Sulfamethazine in Sodium-Hypochlorite

    2012-5-15 · All drug standards (sulfamethoxazole, sulfamethazine, lincomycin, enrofloxacin, and trimethoprim) were of analytical grade and were acquired from Sigma-Aldrich (St. Louis, MO). Additionally, the chemicals used in the chlorination experiments, sodium hypochlorite and sodium sulfate, were of analytical grade and also purchased from Sigma-Aldrich.

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  • Coagulase-Negative Staphylococci

    Antibodies against SesC were able to reduce the S. epidermidis adherence to fibrinogen in vitro as well as in vivo, using a catheter-associated rat infection model (271, 282). Moreover, anti-SesC antibodies were able to decrease the number of S. epidermidis cells in biofilms in vivo.

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  • Improved detection techniques for foodborne pathogens ...

    2009-10-27 · sulfamethoxazole) on spectra was determined. The FTIR technique can be used to discriminate bacteria subjected to different inactivation treatments and possibly to assess the degree of cell injury caused by each treatment. FTIR methods were able to differentiate live and dead cells of E. coli O157:H7 as well as quantify live cells of E. coli ...

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  • 797 PHARMACEUTICAL COMPOUNDING—STERILE

    2019-11-22 · 10. STERILIZATION AND DEPYROGENATION 10.1 Depyrogenation 10.2 Sterilization by Filtration 10.3 Sterilization by Steam Heat 10.4 Sterilization by Dry Heat 11. MASTER FORMULATION AND COMPOUNDING RECORDS 11.1 Creating Master Formulation Records 11.2 Creating Compounding Records 12. RELEASE INSPECTIONS AND TESTING 12.1 Visual Inspection 12.2 ...

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  • Research Paper Iron oxide nanozyme suppresses ...

    2018-12-19 · sterilization in an autoclave (121 oC, 30 min) still showed comparable catalytic activity compared to IONzymes (without sterllization treatment). The Kcat values for sterilized IONzymes to H 2O 2 and TMB were 1.5 × 105/s and 2.1 × 105/s, respectively (Table S1 and S2), suggesting that IONzymes were quite stable and could be readily used for the

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  • Final Report Summary - NOVO (Novel approaches for ...

    2012-1-1 · The CDH coated silicone catheters were also incubated in artificial urine for 16 days at 37 °C in order to test their long term stability in a realistic environment. The enzyme was able to retain 20% of its activity over the entire period which shows again that this system is robust enough to apply it in the coating of the urinary catheters.

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  • Sterilization process for pharmaceutical suspensions ...

    1997-12-19 · Procedures for sterilizing suspensions include ethylene oxide exposure, and the wet steam method in combination with saturated sodium chloride, cited below in U.S. Pat. No. 3,962,430. As previously mentioned, the ethylene oxide method is being phased out, and so new methods are sought to sterilize suspensions.

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  • Paracoccidioidomycosis: reduction in fungal load and ...

    2003-6-28 · Mice were )1 day was able to significantly diminish the fungal load killed at day 150 or 180 p.i., and their organs (spleen, epiploon, )1 )1 in the spleen and lungs, while the 30 mg kg day dose liver and lungs) were aseptically collected and grinded in a tissue grind.

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  • Performance of extraction methods for extracellular

    2019-11-2 · Subsamples were collected haphazardly within a 100 m 2 area, each separated by at least 2 m. The subsamples were kept on ice, returned to the laboratory, and the top 8 mm of the sediment from each subsample was collected by pushing the core with a plunger until the correct amount was above the syringe and “cut” with a sterile plastic knife.

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  • Lab 19: Use of Chemical Agents to Control of ...

    2021-3-19 · a. The term disinfectant is used for an agent used to disinfect inanimate objects or surfaces but is generally to toxic to use on human tissues.. b. The term antiseptic refers to an agent that kills or inhibits growth of microbes but is safe to use on human tissue.. c. The term sanitizer describes an agent that reduces, but may not eliminate, microbial numbers to a safe level.

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  • The Fate of Sulfamethazine in Sodium-Hypochlorite

    2012-5-15 · All drug standards (sulfamethoxazole, sulfamethazine, lincomycin, enrofloxacin, and trimethoprim) were of analytical grade and were acquired from Sigma-Aldrich (St. Louis, MO). Additionally, the chemicals used in the chlorination experiments, sodium hypochlorite and sodium sulfate, were of analytical grade and also purchased from Sigma-Aldrich.

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  • Improved detection techniques for foodborne pathogens ...

    2009-10-27 · sulfamethoxazole) on spectra was determined. The FTIR technique can be used to discriminate bacteria subjected to different inactivation treatments and possibly to assess the degree of cell injury caused by each treatment. FTIR methods were able to differentiate live and dead cells of E. coli O157:H7 as well as quantify live cells of E. coli ...

    Get Price
  • Waterborne Elizabethkingia meningoseptica in Adult ...

    2021-7-14 · patients in whom multiple organisms were identified, we were able to identify case-patients with monomicrobial E. meningoseptica acquisition and, in this subgroup, analyze the trend in clinico-physiologic parameters in the 48 hours before and after acquisition. Three systemic inflammatory response syndrome (SIRS) parameters were investigated

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  • Mineralization of Antibiotics in Wastewater Via ...

    2021-5-16 · Antibiotics are among the prominent class of pharmaceuticals considered emerging pollutants. This class of compounds has found its way into diverse arrays of water bodies in the environment due to their incomplete decomposition, and also the indiscriminate disposal of pharmaceutical waste from industries, farms, and medical centers. This is often through soluble …

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  • 797 PHARMACEUTICAL COMPOUNDING—STERILE

    2019-11-22 · 10. STERILIZATION AND DEPYROGENATION 10.1 Depyrogenation 10.2 Sterilization by Filtration 10.3 Sterilization by Steam Heat 10.4 Sterilization by Dry Heat 11. MASTER FORMULATION AND COMPOUNDING RECORDS 11.1 Creating Master Formulation Records 11.2 Creating Compounding Records 12. RELEASE INSPECTIONS AND TESTING 12.1 Visual Inspection 12.2 ...

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  • Should Asymptomatic Bacteriuria Be Systematically

    2016-4-18 · It is predictable that even if we were able to detect and treat every episode of AB in the usual clinical practice setting, urine sterilization would be achieved in fewer than half of the occasions. Of note, 14.0% of the patients allocated to the control group had persistent AB caused by the same uropathogen for >6 mo without developing symptoms.

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  • Final Report Summary - NOVO (Novel approaches for ...

    2012-1-1 · The CDH coated silicone catheters were also incubated in artificial urine for 16 days at 37 °C in order to test their long term stability in a realistic environment. The enzyme was able to retain 20% of its activity over the entire period which shows again that this system is robust enough to apply it in the coating of the urinary catheters.

    Get Price
  • Paracoccidioidomycosis: reduction in fungal load and ...

    2003-6-28 · Mice were )1 day was able to significantly diminish the fungal load killed at day 150 or 180 p.i., and their organs (spleen, epiploon, )1 )1 in the spleen and lungs, while the 30 mg kg day dose liver and lungs) were aseptically collected and grinded in a tissue grind.

    Get Price
  • Sterilization process for pharmaceutical suspensions ...

    1997-12-19 · Procedures for sterilizing suspensions include ethylene oxide exposure, and the wet steam method in combination with saturated sodium chloride, cited below in U.S. Pat. No. 3,962,430. As previously mentioned, the ethylene oxide method is being phased out, and so new methods are sought to sterilize suspensions.

    Get Price
  • (PDF) Paracoccidioidomycosis: reduction in fungal

    Mice were brasiliensis inoculation, the treatment of 150 mg kg)1 killed at day 150 or 180 p.i., and their organs (spleen, epiploon, day)1 was able to significantly diminish the fungal load liver and lungs) were aseptically collected and grinded in a tissue in the spleen and …

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  • Waterborne Elizabethkingia meningoseptica in Adult ...

    2015-12-18 · Abstract. Elizabethkingia meningoseptica is an infrequent colonizer of the respiratory tract; its pathogenicity is uncertain. In the context of a 22-month outbreak of E. meningoseptica acquisition affecting 30 patients in a London, UK, critical care unit (3% attack rate) we derived a measure of attributable morbidity and determined whether E. meningoseptica is an emerging nosocomial pathogen.

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  • Endophytic Lifestyle of Global Clones of Extended

    2021-2-9 · Samples were washed in running water and sanitized before the isolation of endophytic bacteria. For surface sterilization, ∼4 g of leaves were immersed sequentially in 70% ethanol (1 min), sodium hypochlorite (2.5% chlorine, 4 min), and 70% ethanol (30 s), and then washed three times in sterile distilled water . Aliquots of the sterile water ...

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  • Identification of a novel bZIP transcription factor in ...

    After 5 min surface sterilization in 10 % NaClO, seeds were rinsed five times with sterile water and stratified at 4 °C for 2–4 d before being planted on half-strength Murashige and Skoog (1/2 MS) medium containing 1·5 % sucrose. For the germination test, seeds were grown on 1/2 MS medium with or without 0·5 μ m ABA for 10 d.

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  • 797 PHARMACEUTICAL COMPOUNDING—STERILE

    2019-11-22 · 10. STERILIZATION AND DEPYROGENATION 10.1 Depyrogenation 10.2 Sterilization by Filtration 10.3 Sterilization by Steam Heat 10.4 Sterilization by Dry Heat 11. MASTER FORMULATION AND COMPOUNDING RECORDS 11.1 Creating Master Formulation Records 11.2 Creating Compounding Records 12. RELEASE INSPECTIONS AND TESTING 12.1 Visual Inspection 12.2 ...

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  • nMAT4, a maturase factor required for nad1

    2014-2-8 · Similarly to the knockout line, nmat4-2 plants demonstrated reduced germination and altered growth phenotypes (Figure 2b), but were still able to set some viable seeds. The milder phenotypes of nmat4 - 2 mutants, compared with those seen in nmat4 - 1 (Figure 2 b), are probably associated with incomplete silencing of nMAT4 gene expression, as ...

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  • Sphingomonas paucimobilis- Infectious Disease and ...

    Sphingomonas paucimobilis was initially known as CDC Group IIk, biotype 1, subsequently receiving its own taxonomic status in 1977, when it was named Pseudomonas paucimobilis ( 14 ). In 1990 the bacteria was placed into its own genus Sphingomonas and as the type strain of the genus ( 36 ). It shares biochemical properties, fatty acid content ...

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  • DailyMed - DEXTROSE injection, solution

    2020-6-17 · Dextrose Injection 20%, 30%, 40%, 50%, and 70% USP are sterile, non-pyrogenic, hypertonic solutions of dextrose in single-dose, partial-fill, flexible containers. 500 mL fill volume in 1000 mL flexible container. •. 20% (0.2 grams/mL): 20 grams of dextrose hydrous per 100 mL.

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  • EIN3 and ORE1 Accelerate Degreening during

    2015-7-28 · After surface sterilization, imbibed seeds were stratified for 3 days at 4°C to synchronize germination. Plants were grown in an environmentally controlled chamber at 22°C-24°C, at light intensity of approximately 120 μmol m-2 s-1 under a 16-hr light/8-hr dark photoperiod.

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  • Thermal Processing - Fo Value calculation - IFSQN

    2009-7-2 · Page 1 of 2 - Thermal Processing - Fo Value calculation - posted in Food Technology: Dear all Please define underneath term in retort processing Presently I switch Bakery to RTE industry. 1.Fo value calculation. 2.Z value calculation 3.How to conduct HD test. 4.How to conduct HP test.and what is base of these. Thanks, Tripathi

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  • Limitations in predicting radiation-induced

    2019-6-6 · While the NSRL exposures were used as a terrestrial radiation study arm to examine radiation effects on the vitamin, it is noteworthy that the NSRL exposures were monoenergetic exposures of 0.1 ...

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  • The Fate of Sulfamethazine in Sodium-Hypochlorite

    2012-5-15 · Pharmaceutical compounds represent a rapidly emerging class of environmental contaminants. Such compounds were recently classified by the U.S. Geological Survey, including several antibiotics. An LC-MS/MS screening method for the top five antibiotics in drinking water was developed and validated using a Shimadzu LC-MS-IT-TOF. The separation was performed using a Waters …

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  • Waterborne Elizabethkingia meningoseptica in Adult ...

    2015-12-18 · Abstract. Elizabethkingia meningoseptica is an infrequent colonizer of the respiratory tract; its pathogenicity is uncertain. In the context of a 22-month outbreak of E. meningoseptica acquisition affecting 30 patients in a London, UK, critical care unit (3% attack rate) we derived a measure of attributable morbidity and determined whether E. meningoseptica is an emerging nosocomial pathogen.

    Get Price
  • Identification of a novel bZIP transcription factor in ...

    After 5 min surface sterilization in 10 % NaClO, seeds were rinsed five times with sterile water and stratified at 4 °C for 2–4 d before being planted on half-strength Murashige and Skoog (1/2 MS) medium containing 1·5 % sucrose. For the germination test, seeds were grown on 1/2 MS medium with or without 0·5 μ m ABA for 10 d.

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  • The PIN1 family gene PvPIN1 is involved in auxin

    2014-10-10 · The cycle thresholds were determined using a Bio-Rad CFX96 sequence detection system with a volume of 25 μL containing 12.5 μL ofTaqMan Master Mix SYBR Premix ExTaq (Takara). The cycling conditions were as follows: 95 °C for 10 min, followed by …

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  • Process Validation (PV)

    2015-11-24 · process variables were used during manufacturing. The study should be robust enough to prove that those ranges of variables were able to produce proposed specification. For Media Fills There is a requiremen t to compare the parameters used for production filling and for media fill s. 8

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  • Microbial Degradation of Chlorogenic Acid by a ...

    2016-4-11 · In order to elucidate the metabolism of chlorogenic acid by environmental microbes, a strain of Sphingomonas sp. isolated from tobacco leaves was cultured under various conditions, and chlorogenic acid degradation and its metabolites were investigated. The strain converting chlorogenic acid was newly isolated and identified as a Sphingomonas sp. strain by 16S rRNA sequencing.

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  • DailyMed - GANCICLOVIR injection, solution

    2018-6-1 · Protein, ash, moisture, carbohydrate, and fat contents of the premix-B formula were 23.51%, 2.44%, 7.31%, 71.02%, 4.28%, respectively, which met the SNI requirements. Overall, this study clearly showed that it was possible to make otak-otak with less complicated process and the product did not have any negative results on sensory perception.

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  • IOP Conference Series: Earth and Environmental

    Sphingomonas paucimobilis was initially known as CDC Group IIk, biotype 1, subsequently receiving its own taxonomic status in 1977, when it was named Pseudomonas paucimobilis ( 14 ). In 1990 the bacteria was placed into its own genus Sphingomonas and as the type strain of the genus ( 36 ). It shares biochemical properties, fatty acid content ...

    Get Price
  • The Fate of Sulfamethazine in Sodium-Hypochlorite

    2012-5-15 · Pharmaceutical compounds represent a rapidly emerging class of environmental contaminants. Such compounds were recently classified by the U.S. Geological Survey, including several antibiotics. An LC-MS/MS screening method for the top five antibiotics in drinking water was developed and validated using a Shimadzu LC-MS-IT-TOF. The separation was performed using a Waters …

    Get Price
  • Role of Folate Antagonists in the Treatment of

    Patients were injection drug users, most of them had endovascular infection, and 47% of the S. aureus isolates from these patients were MRSA. Vancomycin was superior to TMP-SMZ in terms of the duration of bacteremia (6.7 vs. 4.3 days), sterilization of wound culture (5.8 vs. 3.8 days), duration of fever, and failure rates (6 of 43 with TMP-SMZ ...

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  • The PIN1 family gene PvPIN1 is involved in auxin

    2014-10-10 · The cycle thresholds were determined using a Bio-Rad CFX96 sequence detection system with a volume of 25 μL containing 12.5 μL ofTaqMan Master Mix SYBR Premix ExTaq (Takara). The cycling conditions were as follows: 95 °C for 10 min, followed by …

    Get Price
  • Genetic diversity and characteristics of high-level ...

    2020-12-7 · The recent emergence and dissemination of high-level mobile tigecycline resistance Tet(X) challenge the clinical effectiveness of tigecycline, one of the last-resort therapeutic options for complicated infections caused by multidrug-resistant Gram-negative and Gram-positive pathogens. Although tet(X) has been found in various bacterial species, less is known about phylogeographic …

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  • Exclusionary interactions among diverse fungi

    2013-4-1 · Re-isolations were carried out after surface-sterilization ‘Method I’. In a third experiment, Botrytis , Cladosporium , and Fusarium inoculations of flower heads of six plants were followed by milder surface sterilization of seeds (Method II: immersion in 70% ethanol, plus Tween, for 5 min, followed by three rinses in SDW).

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  • Mycobacterium marinum - Infectious Disease and ...

    Mycobacterium marinum, a non-tuberculous pathochromogen with an intermediate growth rate between rapidly and slowly growing mycobacteria, belongs to group I of the Runyon classification ( 57a ). M. marinum grows optimally at 28° to 32° C (within 2-3 weeks), while fails to grow on primary isolation at 37°C, a feature that distinguishes this ...

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  • SUMMARY OF SAFETY AND EFFECTIVENESS DATA

    2003-5-1 · than on primary safety outcomes. A total of 358 eyes were enrolled (357 actually treated). There were 290 eyes (233 non-monovision and 57 monovision) with 6 months of follow-up data. Based on the sample size calculations provided in the original protocol, at least 196 non-monovision-treated eyes would be required to demonstrate effectiveness.

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  • IOP Conference Series: Earth and Environmental

    2018-6-1 · Protein, ash, moisture, carbohydrate, and fat contents of the premix-B formula were 23.51%, 2.44%, 7.31%, 71.02%, 4.28%, respectively, which met the SNI requirements. Overall, this study clearly showed that it was possible to make otak-otak with less complicated process and the product did not have any negative results on sensory perception.

    Get Price
  • Recent progress in biochar-supported photocatalysts ...

    Recent progress in biochar-supported photocatalysts: synthesis, role of biochar, and applications. Md Manik Mian ab and Guijian Liu * ab a CAS Key Laboratory of Crust-Mantle Materials and Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China. E-mail: [email protected]; Fax: +86 551 63621485; Tel: +86 551 63603714 b State Key ...

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  • ACaseof Serratiamarcescens

    2019-7-30 · hemoptysis were resolved, blood tests remained within normal limits, including inflammation markers such as C-reactive protein (CRP) and WBC, and multiple sub-sequent blood culture results came back negative. A new transesophageal echocardiogram was performed 20days afterthepreviousone,withoutfurtherdeterioration.

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  • Coagulase-Negative Staphylococci

    INTRODUCTION. It was 20 years ago that Kloos and Bannerman updated our knowledge on the clinical significance of coagulase-negative staphylococci (CoNS), following a review, 6 years previously, of their laboratory, clinical, and epidemiological aspects by Pfaller and Herwaldt (), both in this journal.Although the pathogenic potential of CoNS had become accepted by the end of the 1980s, most of ...

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  • Frontiers | Prevalence, Virulence Genes, Antimicrobial ...

    2013-4-1 · Re-isolations were carried out after surface-sterilization ‘Method I’. In a third experiment, Botrytis , Cladosporium , and Fusarium inoculations of flower heads of six plants were followed by milder surface sterilization of seeds (Method II: immersion in 70% ethanol, plus Tween, for 5 min, followed by three rinses in SDW).

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  • Exclusionary interactions among diverse fungi

    2018-6-6 · Glutaraldehyde possesses unique characteristics that render it one of the most effective protein crosslinking reagents. It can be present in at least 13 different forms depending on solution conditions such as pH, concentration, temperature, etc. Substantial literature is found concerning the use of glutaraldehyde for protein immobilization, yet there is no agreement about the main reactive ...

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  • Glutaraldehyde: behavior in aqueous solution,

    2003-5-1 · than on primary safety outcomes. A total of 358 eyes were enrolled (357 actually treated). There were 290 eyes (233 non-monovision and 57 monovision) with 6 months of follow-up data. Based on the sample size calculations provided in the original protocol, at least 196 non-monovision-treated eyes would be required to demonstrate effectiveness.

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  • SUMMARY OF SAFETY AND EFFECTIVENESS DATA

    2020-6-19 · Plates were incubated at 27°C for 3 days. No growth was detected for any plant. All sterilization steps were carried out in a laminar flow cabinet in sterile conditions. Plant organs were ground with sterile mortar and pestle using autoclaved peptone water (0.9 mL per tissue gram) to recover the microorganisms.

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  • Frontiers | Culturable Bacterial Endophytes From ...

    Recent progress in biochar-supported photocatalysts: synthesis, role of biochar, and applications. Md Manik Mian ab and Guijian Liu * ab a CAS Key Laboratory of Crust-Mantle Materials and Environments, School of Earth and Space Sciences, University of Science and Technology of China, Hefei 230026, China. E-mail: [email protected]; Fax: +86 551 63621485; Tel: +86 551 63603714 b State Key ...

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  • Recent progress in biochar-supported photocatalysts ...

    2017-6-14 · After sterilization, SEM medium that had been 10-fold diluted was cooled to 40–50 °C, after which a series of stock organic acid solutions dissolved in double distilled water were filtered ...

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  • The role of organic acids on microbial deterioration in ...

    2018-6-8 · mately 0.5g fresh weight) frozen in liquid nitrogen were ground with a mortar and pestle and homogenized, and extracted with 10ml of 90% methanol and then with 10ml of 100% methanol. These two extracts were combined, and 2ml of the resulting extract was dried at 40 C. The SA and SAG (free SA and SA-glucoside) content was measured as previously ...

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  • Effects of Colonization of a Bacterial Endophyte ...

    A premix for preparing an emulsion composition, which makes it possible to easily prepare a food containing a highly unsaturated fatty acid such as DHA or EPA, can be obtained by mixing a highly unsaturated fatty acid-containing fat or oil with a specific fat or oil and then mixing the resultant mixture with a preset amount of an aqueous ...

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  • Broad-spectrum in vitro antibacterial activities of clay ...

    2007-12-10 · To ensure that the clay samples were sterilized after autoclaving and maintained sterilization during storage, negative control growth experiments with clay minerals in LB broth were performed several times throughout the course of the study. Incubation of the bacteria–clay mixtures was performed as follows: E. coli, S.

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  • Activation of AMPK sensitizes medulloblastoma to ...

    2021-2-24 · The cDNA was amplified with SYBR Premix (Roche, Basel, Switzerland) using a Light Cycler 96 Real-Time PCR System (Roche, Basel, Switzerland). The PCR cycling parameters were 95°C for 10 min, then 95°C for 10 s, 60°C for 10 s, and 72°C for 20 s repeated 40 times. A single primer for each sample was carried out in triplicate.

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  • MAUDE Adverse Event Report: BAYER HEALTHCARE

    2014-12-12 · MAUDE Adverse Event Report: BAYER HEALTHCARE LLC ESSURE DEVICE, OCCLUSION, TUBAL, CONTRACEPTIVE. The event 'abscesses on arms' was recoded to (b) (4) 'abscesses of skin'. Follow-up information received on 01-apr-2014: this case is now potential legal.

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  • The International Pharmacopoeia - WHO

    2021-4-29 · The International Pharmacopoeia (Ph. Int.) constitutes a collection of recommended procedures for analysis and specifications for the determination of pharmaceutical substances and dosage forms that is intended to serve as source material for reference or adaptation by any WHO Member State wishing to establish pharmaceutical requirements. The pharmacopoeia, or any part of …

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  • EIN3 and ORE1 Accelerate Degreening during

    2015-7-28 · After surface sterilization, imbibed seeds were stratified for 3 days at 4°C to synchronize germination. Plants were grown in an environmentally controlled chamber at 22°C-24°C, at light intensity of approximately 120 μmol m-2 s-1 under a 16-hr light/8-hr dark photoperiod.

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  • PREMIX FOR PREPARING EMULSION COMPOSITION,

    A premix for preparing an emulsion composition, which makes it possible to easily prepare a food containing a highly unsaturated fatty acid such as DHA or EPA, can be obtained by mixing a highly unsaturated fatty acid-containing fat or oil with a specific fat or oil and then mixing the resultant mixture with a preset amount of an aqueous ...

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  • JCI - Intestinal development and homeostasis require ...

    2019-4-2 · Moreover, Helios + Foxp3 – CD4 + PP T cells were not able to suppress proliferation of effector T cells , did not secrete IL-4, IFN-γ, or IL-17 after polyclonal stimulation, and were distinct from a subgroup of latency-associated peptide–expressing (LAP-expressing) Tregs, shown to produce IL-10 and TGF-β (Supplemental Figure 3) .

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  • Genome editing of bread wheat using biolistic

    2018-2-1 · This protocol extension describes DNA-free genome editing of bread wheat by delivering in vitro transcripts (IVTs) or ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 by particle bombardment. The ...

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  • If I have allergies, should I be worried about the

    2009-7-2 · Page 1 of 2 - Thermal Processing - Fo Value calculation - posted in Food Technology: Dear all Please define underneath term in retort processing Presently I switch Bakery to RTE industry. 1.Fo value calculation. 2.Z value calculation 3.How to conduct HD test. 4.How to conduct HP test.and what is base of these. Thanks, Tripathi

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