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weikangxing for gastroenteritis using method

  • Chinese Patent Medicine Oral Liquid Weikangxing

    Home > Products > Chinese Patent Medicine Oral Liquid > WeiKangXing . WeiKangXing. WeiKangXing is can prevention and combined treatment of glandular and gastroenteritis, Mycoplasma synovialis, which can effectively reduce the incidence of “gastroenteritis”, improve the uniformity and vaccine immune response.

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  • Syndromic Surveillance of Acute Gastroenteritis

    The French national public health agency (Santé publique France) has used data from the national health insurance reimbursement system (SNDS) to identify medicalised acute gastroenteritis (mAGE) for more than 10 years. This paper presents the method developed to evaluate this system: performance and …

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  • Development of a reverse transcription-PCR-DNA

    Using this method, low levels of NV were detected in stool and both NLV and hepatitis A virus were detected in bivalve mollusks following bioaccumulation. The method also successfully detected NLV in oysters implicated in an outbreak of NLV gastroenteritis. This method dramatically decreases the time needed for analysis and is amenable to …

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  • Development of a colony hybridization method

    2014-9-1 · Vibrio parahaemolyticus is a marine microorganism, recognized as cause of gastroenteritis outbreaks associated with seafood consumption. In this study the development and the in-house validation of a colony hybridization method for the enumeration of total and potentially pathogenic V. parahaemolyticus is reported.

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  • Proteotyping as alternate typing method to

    The aim of this study was to establish a proteotyping scheme using MALDI-TOF MS to offer an alternative to sequence-based methods. A total of 97 clade-representative C. coli isolates were analyzed by MALDI-TOF-based intact cell mass spectrometry (ICMS) and evaluated to establish a C. coli proteotyping scheme. MLST was used as reference method.

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  • Evaluation of the Dako IDEIA norovirus EIA assay

    Methods: A total of 130 faecal specimens from 41 gastroenteritis outbreaks were tested for norovirus by electron microscopy (EM), a two-round multiplex reverse transcription-polymerase chain reaction (RT-PCR) method and the IDEIA norovirus assay. All specimens with sufficient amplified product were sequenced to determine their norovirus genotype.

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  • Optimisation of a PMAxx™-RT-qPCR Assay and

    Human noroviruses are a major cause for gastroenteritis outbreaks. Filter-feeding bivalve molluscs, which accumulate noroviruses in their digestive tissues, are a typical vector for human infection. RT-qPCR, the established method for human norovirus detection in food, does not allow discrimination between infectious and non-infectious viruses and can …

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  • DETECTION OF A NEW VIRUS BY ELECTRON

    1974-2-2 · The method of differential centrifugation for extraction of fæces is simple, quick, and more sensitive than duodenol biopsy for detecting the virus. Combining results from duodenal mucosa and fæces, orbivirus particles were observed by electron microscopy in 81% of children with sporadic gastroenteritis in Melbourne.

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  • Detection and genetic characterization of

    Of 972 stool samples collected from infants and children with acute gastroenteritis in six different regions (Hokkaido, Tokyo, Shizuoka, Kyoto, Osaka, and Saga) of Japan during 2-year period from July 2014 to June 2016, 63 samples (6.5%) determined to be positive for enterovirus by multiplex RT-PCR were subjected to genotype determination based on the partial VP1 region using the CODEHOP method.

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  • Development of Poly (A)-Tailed Universal

    Development of Poly (A)-Tailed Universal Reverse Transcription PCR Method forSequence-Independent Amplification of Rearranged Rotavirus. Ahmadi E(1), Soleimanjahi H(1), Sadeghizadeh M(2), Teimoori A(3). Author information: (1)Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

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  • Universal extraction method for gastrointestinal

    A universal stool extraction method for recovery of nucleic acids (NAs) from gastrointestinal pathogens was developed to support rapid diagnostics for the London 2012 Olympics. The method involved mechanical disruption (bead beating) of the stools, followed by automated extraction and detection using real-time PCR.

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  • An Overview of the Molecular Methods in the

    2020-3-24 · Gastrointestinal infectious diseases are very common worldwide and an important cause of morbidity and mortality, particularly in infants in developing countries. Diarrhea and other intestinal infections are caused by a wide range of bacteria, viruses, protozoa, and parasites. Conventional diagnosis of these infections is performed by …

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  • Development of a colony hybridization method

    2014-9-1 · Vibrio parahaemolyticus is a marine microorganism, recognized as cause of gastroenteritis outbreaks associated with seafood consumption. In this study the development and the in-house validation of a colony hybridization method for the enumeration of total and potentially pathogenic V. parahaemolyticus is reported.

    Get Price
  • Evaluating community pharmacy practice in Qatar

    Viral gastroenteritis is generally a self-limiting watery diarrheal illness with no requirement for antibiotics. In this condition, there is a possibility for irrational dispensing by community pharmacists. Original Research Evaluating community pharmacy practice in Qatar using simulated patient method: acute gastroenteritis management

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  • Evaluation of a New Environmental Sampling

    2015-8-7 · Inanimate surfaces are regarded as key vehicles for the spread of human norovirus during outbreaks. ISO method 15216 involves the use of cotton swabs for environmental sampling from food surfaces and fomites for the detection of norovirus genogroup I (GI) and GII. We evaluated the effects of the virus drying time (1, 8, 24, or …

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  • Manual of rotavirus detection and characterization

    2019-10-24 · Manual of rotavirus detection and characterization methods WHO/IVB/08.17 ORIGINAL: ENGLISH Immunization, Vaccines and Biologicals

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  • Detection and genetic characterization of

    1. Infect Genet Evol. 2018 Jul;61:16-19. doi: 10.1016/j.meegid.2018.03.009. Epub 2018 Mar 11. Detection and genetic characterization of enterovirus strains circulating among children with acute gastroenteritis in Japan during 2014-2016.

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  • DETECTION OF A NEW VIRUS BY ELECTRON

    1974-2-2 · The method of differential centrifugation for extraction of fæces is simple, quick, and more sensitive than duodenol biopsy for detecting the virus. Combining results from duodenal mucosa and fæces, orbivirus particles were observed by electron microscopy in 81% of children with sporadic gastroenteritis in Melbourne.

    Get Price
  • Development of Poly (A)-Tailed Universal

    Development of Poly (A)-Tailed Universal Reverse Transcription PCR Method for Sequence-Independent Amplification of Rearranged Rotavirus. Ahmadi E(1), Soleimanjahi H(1), Sadeghizadeh M(2), Teimoori A(3). Author information: (1)Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

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  • Evaluating community pharmacy practice in

    2016-10-1 · This method is also simple and is a method for assessing the community pharmacists’ dispensing practices.12,13 The simulated patient method, which is also described in literature as the mystery shopper technique, is a method that can be effectively used to study the practice perspectives and professional behaviors.

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  • Universal extraction method for gastrointestinal

    A universal stool extraction method for recovery of nucleic acids (NAs) from gastrointestinal pathogens was developed to support rapid diagnostics for the London 2012 Olympics. The method involved mechanical disruption (bead beating) of the stools, followed by automated extraction and detection using real-time PCR.

    Get Price
  • Development of a reverse transcription-PCR-DNA

    Using this method, low levels of NV were detected in stool and both NLV and hepatitis A virus were detected in bivalve mollusks following bioaccumulation. The method also successfully detected NLV in oysters implicated in an outbreak of NLV gastroenteritis. This method dramatically decreases the time needed for analysis and is amenable to ...

    Get Price
  • Gastroenteritis | NHS inform

    2020-3-25 · Gastroenteritis is a very common condition that causes diarrhoea and vomiting. It's usually caused by a bacterial or viral tummy bug. It affects people of all ages, but is particularly common in young children. Most cases in children are caused by a virus called rotavirus. Cases in adults are usually caused by norovirus (the 'winter vomiting ...

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  • Phylogenetic and recombination analysis of

    2011-2-24 · Phylogenetic tree was constructed by the neighbor-joining method, Kimura 2-parameter model with 1,000 bootstrap replicates, by using MEGA 4.1 package. Black dots designate reference strains, black triangles designate sequences from control group and the others were sequences generated from the gastroenteritis children in the present …

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  • Meta‐analysis: Lactobacillus GG for treating

    2013-7-10 · Introduction. Previously, we have shown in a meta‐analysis that Lactobacillus rhamnosus GG (LGG) is effective in treating acute gastroenteritis (AGE) in children. 1 In brief, compared with controls, although LGG had no effect on the total stool volume, its use was associated with a significant reduction in diarrhoea duration, particularly of …

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  • The N-Terminal Domain of Spike Protein Is Not the

    2019-3-30 · viruses Article The N-Terminal Domain of Spike Protein Is Not the Enteric Tropism Determinant for Transmissible Gastroenteritis Virus in Piglets Gang Wang 1,2, Rui Liang 1,2, Ziwei Liu 1,2, Zhou Shen 1,2, Jiale Shi 1,2, Yuejun Shi 1,2, Feng Deng 1,2, Shaobo Xiao 1,2, Zhen F. Fu 1,2,3 and Guiqing Peng 1,2,4,* 1 State Key Laboratory of …

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  • Hand sanitiser no match for gastro – use soap

    2021-4-19 · People using hand sanitiser as a substitute for washing their hands with soap and water have been partly blamed for a spike in viral gastroenteritis.

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  • See tab below for CDC response Contributions on

    2020-5-21 · (LLC-MCK2) (ATCC # CCL-7.1), testing using the OECD method and ASTM E1053 method. NL63 is available from BEI Resources. Will be appropriate surrogate when virus receptors are the object studies as it utilizes the same receptors as SARS. Feline infectious peritonitis virus ATCC VR-2009, ATCC VR-2126, ATCC VR-1812 ss RNA, …

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  • US Patent for Methods for producing

    2002-4-19 · A helper cell for producing an infectious, replication defective, coronavirus (or more generally nidovirus) particle cell comprises (a) a nidovirus permissive cell; (b) a nidovirus replicon RNA comprising the nidovirus packaging signal and a heterologous RNA sequence, wherein the replicon RNA further lacks a sequence encoding at least …

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  • Data-driven outbreak forecasting with a simple

    2016-12-1 · The parabolas plotted in the figures were selected using the automated parameter approximation method. Inspection of these plots reveals that they capture the time course of the cumulative number of cases fairly well (right panel of each figure and as depicted in panel 5 of the schematic of Fig. 1). For very noisy data (e.g. left panels of …

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  • Development of a reverse transcription-PCR-DNA

    Using this method, low levels of NV were detected in stool and both NLV and hepatitis A virus were detected in bivalve mollusks following bioaccumulation. The method also successfully detected NLV in oysters implicated in an outbreak of NLV gastroenteritis. This method dramatically decreases the time needed for analysis and is amenable to ...

    Get Price
  • A Comparative Study of Four Methods for Detecting

    2013-5-8 · 10 24 Acute gastroenteritis Convolutions Moderately -+ 2 2 abnormal 11 14 Acute gastroenteritis Leaves Moderately + + + 2 2 abnormal 12 23 Acute gastroenteritis Convolutions Mildly + + + 3 2 abnormal ' +, presence of Giardia lamblia; -, absence of Giardia lamblia. /, Direct fecal smear method.

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  • A validated spectrofluorimetric method for the ...

    2013-5-23 · A novel and sensitive spectrofluorimetric method was designed and validated for the determination of NF in pharmaceutical formulation. The method was based upon the formation of a highly fluorescent coumarin compound by the reaction between NF and ethylacetoacetate (EAA) using sulfuric acid as catalyst.

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  • Accuracy and comparison of two rapid multiplex PCR

    Changfi J, etflal BMJ Open Gastro 20218:e000553 doi:101136bmjgast2020000553 1 Accuracy and comparison of two rapid multiplex PCR tests for gastroenteritis pathogens: a systematic review and meta- analysis Li- Jun Chang ,1 Chun-Jen Hsiao ,1 Bing Chen ,2 Tzu-u LiuY ,3 Julia Ding ,4 Wan-Ting Hsu ,5,6 Victor Su-Ortiz ,7,8 Szu-a ChenT ,5

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  • Establishment of a dual mode

    2019-8-15 · Campylobacter jejuni (C. jejuni) is considered one of the most common cause of human gastroenteritis.Aiming to detect C. jejuni in food products rapidly and sensitively, a dual mode lateral flow assay, based on the peroxidase mimicking and surface enhanced Raman scattering (SERS) enhancement properties of platinum …

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  • Enhanced Reverse Transcription-PCR Assay for

    We have developed a one-tube reverse transcription (RT)-PCR method using the real-time TaqMan PCR system for the detection of norovirus genogroup I (NV GGI). By introduction of a novel probe based on locked nucleic acid technology, we enhanced the sensitivity of the assay compared to those of conventional TaqMan probes. The sensitivity of the NV …

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  • ISO/DIS 15216-2(en), Microbiology of the food

    ISO 15216-1, Microbiology of the food chain — Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR — Part 1: Method for quantification [3] ISO 17468, Microbiology of the food chain — Technical requirements and guidance on establishment or revision of a standardized reference method [4]

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  • Pathogens | Free Full-Text | Fecal Source

    Immunochromatography is the preserve of research laboratories as a laboratory technique. Near-patient testing, however, utilizes commercially produced ‘sticks’ which use immunochromatography. The principle is that the sample, e.g. urine, is applied to the stick which is then developed, e.g. by capillary attraction, the analyte of interest binding …

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  • Efficacy of Ondansetron on Vomiting Due to

    2014-1-7 · Acute gastroenteritis is a common disease especially in children. With bronchiolitis and influenza, she participated widely in weight of winter epidemics that causes problems every year our health care system, particularly in the pediatric emergency and inpatient since they are the second leading cause of hospitalization in …

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  • Comparative evaluation of different molecular

    Antiemetics in Children With Acute Gastroenteritis: A Meta-analysis Laura F. Niño-Serna, MD, MSc,a,b Jorge Acosta-Reyes, MD, MSc,c Areti-Angeliki Veroniki, PhD,d,e,f Ivan D. Florez, MD, MSca,g CONTEXT: Several antiemetics have been used in children with acute gastroenteritis. However, abstract there is still controversy over their use.

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  • Antiemetics in Children With Acute Gastroenteritis: A

    2018-5-2 · This study aimed to compare the FECPAKG2 and the McMaster techniques for counting of gastrointestinal nematode eggs in the faeces of alpacas using two floatation solutions (saturated sodium chloride and sucrose solutions). Faecal eggs counts from both techniques were compared using the Lin’s concordance correlation coefficient …

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  • Comparison of McMaster and FECPAK G2

    2019-8-15 · Campylobacter jejuni (C. jejuni) is considered one of the most common cause of human gastroenteritis.Aiming to detect C. jejuni in food products rapidly and sensitively, a dual mode lateral flow assay, based on the peroxidase mimicking and surface enhanced Raman scattering (SERS) enhancement properties of platinum …

    Get Price
  • Establishment of a dual mode

    1987-2-1 · Micro-method for neutralization test of transmissible gastroenteritis virus using porcine kidney cell line, CPK cells. Komaniwa H, Fukusho A, Shimizu Y. Nihon Juigaku zasshi. The Japanese Journal of Veterinary Science, 01 Feb 1987, 49(1): 141-144 DOI: 10.1292/jvms1939.49 ...

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  • Micro-method for neutralization test of

    2019-10-24 · vi Nicola A. Page MRC Diarrhoeal Pathogens Research Unit (DPRU) University of Limpopo (Medunsa Campus) PO Box 173, Medunsa 0204, South Africa The following members of the “Rotavirus Experts External Advisory

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  • WHO | WHO/IRIS - WHO | World Health Organization

    2000-9-21 · The results of data analyses by computer programs described in the section on data analysis were verified using data commonly obtained from effluent toxicity tests. However, these computer programs may not be applicable ... Plotted data and fitted line for graphical method, using all-or-nothing data..... 80 8. Example of input for computer ...

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  • EPA/600/4-90/027F August 1993 METHODS FOR

    2017-9-21 · Porcine Deltacoronavirus (PDCoV) is a newly emerged enteropathogenic coronavirus that causes diarrhea and mortality in neonatal piglets. PDCoV has spread to many countries around the world, leading to significant economic losses in the pork industry. Therefore, a rapid and sensitive method for detection of PDCoV in clinical …

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  • A simple and rapid identification method for

    2020-12-21 · Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J. Clin. Microbiol.42:1564-1569.

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  • 'Optimization of sampling and quantification

    Norovirus is the most common pathogen to cause acute gastroenteritis in the world. Symptoms of acute gastroenteritis include vomiting and/or diarrhea, along with fever, abdominal pain, and malaise. Annually, norovirus causes 685 million cases of acute gastroenteritis and 200,000 deaths, worldwide. Among the 685 million cases occurring …

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  • Gastroenteritis in Children: Part II. Prevention and ...

    2012-6-1 · An accelerated method of oral rehydration therapy for ... When rehydrating a child with gastroenteritis using an oral rehydration solution, an …

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  • Water, sanitation, hygiene, and waste management

    2021-5-4 · gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV). Water, sanitation, hygiene, and waste management for the COVID-19 virus: interim guidance -2- ... method is using an alcohol -based hand rub for 20−30 seconds using the appropriate technique. 22. When hands are visibly

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  • Continuous Enteral Rehydration by Nasogastric

    2020-9-18 · When oral rehydration is impossible, enteral rehydration via the nasogastric route has been the recommended method of rehydration since 2008 by ESPGHAN ( European Society for Paediatric Gastroenterology Hepatology and Nutrition ), for children with acute gastroenteritis. However, these recommendations are rarely followed in …

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  • A comparison of the Allplex™ bacterial and viral

    2018-7-28 · A limitation of our study was the use of EM as the comparator method for viral detection in stool samples. As we observed in this study, EM has been found to be less sensitive than molecular methods for diagnosis of viral gastroenteritis [], so use of lab-developed or commercial molecular methods as the comparator would have been preferable.. However, EM was the method …

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  • Molecular Identification of Virulence Genes (agfA

    BACKGROUND AND OBJECTIVE: Gastroenteritis caused by salmonella is common in humans and is regarded as a global public health issue. The aim of this study is to identify agfA and mgtC genes in salmonella typhimurium strains isolated from stool samples using multiplex PCR method and to determine the resistance patterns of these strains.

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  • Oral Ondansetron for Gastroenteritis in a

    2010-9-17 · 2005) and 0.79 pppy for ‘acute gastroenteritis’ in Americans of all ages (Mead et al. 1999) − i.e., in the developing-country range shown in the table. Source: Mathers et al. (2002). In low- and middle-income countries diarrhoeal diseases caused a total DALY loss of 59 million in 2001 (Lopez et al. 2006, Ezzati

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  • Discussion PaPer: oPtions for uPDatinG tHe 2006

    2006-5-1 · Jung, K, Chae, C: 2005, RT-PCR-based dot blot hybridization for the detection and differentiation between porcine epidemic diarrhea virus and transmissible gastroenteritis virus in fecal samples using a non-radioactive digoxigenin cDNA probe.

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  • Multiplex Reverse Transcription-PCR for Rapid

    Two trials were conducted against natural and experimentally induced parasitic gastroenteritis in sheep and goats using an in-feed formulation of albendazole to evaluate its therapeutic and prophylactic efficacy. In the first trial, albendazole was incorporated in feed pellets to deliver an average daily dose of 0.7 mg/kg body weight in order to …

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  • Gastroenteritis in Children: Part II. Prevention and ...

    2012-6-1 · An accelerated method of oral rehydration therapy for ... When rehydrating a child with gastroenteritis using an oral rehydration solution, an …

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  • 'Optimization of sampling and quantification

    Norovirus is the most common pathogen to cause acute gastroenteritis in the world. Symptoms of acute gastroenteritis include vomiting and/or diarrhea, along with fever, abdominal pain, and malaise. Annually, norovirus causes 685 million cases of acute gastroenteritis and 200,000 deaths, worldwide. Among the 685 million cases occurring …

    Get Price
  • Differential Detection of Transmissible

    2001-11-1 · Transmissible gastroenteritis (TGE) and porcine epidemic diarrhea (PED) are highly contagious enteric diseases of piglets. The clinical signs of these diseases are very similar and include watery, yellowish diarrhea. Thus, the effective differential detection of TGE virus and PED virus is required.

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  • Water, sanitation, hygiene, and waste management

    2021-5-4 · gastroenteritis virus (TGEV) and mouse hepatitis virus (MHV). Water, sanitation, hygiene, and waste management for the COVID-19 virus: interim guidance -2- ... method is using an alcohol -based hand rub for 20−30 seconds using the appropriate technique. 22. When hands are visibly

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  • Challenges of Retrospective studies 8March2017 Kim

    2017-4-28 · Hypothesis Formulation and Errors in Research All analytic studies must begin with a clearly formulated hypothesis. The hypothesis must be quantitative and specific (testable with existing data). It must predict a relationship of a specific size. But even with the best formulated hypothesis, two types of errors can occur: Type 1- …

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  • Evaluating community pharmacy practice in

    Evaluating community pharmacy practice in Qatar using simulated patient method: Acute gastroenteritis management. View Open. 800-Article Text-2163-1-10-20161206.pdf (817.2Kb) Date 2016. Author IBRAHIM, Mohamed I. PALAIAN, Subish AL-SULAITI, Fatima EL-SHAMI, Somia. Metadata Show full item record.

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  • Oral Ondansetron for Gastroenteritis in a

    2011-3-18 · Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS), and porcine dermatitis and nephropathy syndrome (PDNS). It has caused heavy losses in global agriculture in recent decades. Rapid detection of PCV2 is very important for the effective prophylaxis and treatment of …

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  • Specific, simple and rapid detection of porcine

    2021-2-1 · The widespread popularity of porcine circovirus type 2(PCV2) has seriously affected the healthy development of the pig industry and caused huge economic losses worldwide. A rapid and reliable method is required for epidemiological investigation and evaluating the effect of immunization. However, the current methods for PCV2 antibody …

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  • A nanobody‐horseradish peroxidase fusion

    2020-11-3 · Rice is a staple food for more than half of the world’s population. Alarmingly, arsenic is a widespread contaminant in rice crops that is getting increased attention as a risk factor in the development of cancer. Studies in recent years have focused on testing various cooking techniques as a way to reduce levels of this heavy metal in brown and …

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  • Gastroenteritis in Children: Part II. Prevention and ...

    2012-6-1 · An accelerated method of oral rehydration therapy for ... When rehydrating a child with gastroenteritis using an oral rehydration solution, an …

    Get Price
  • Molecular Identification of Virulence Genes (agfA

    BACKGROUND AND OBJECTIVE: Gastroenteritis caused by salmonella is common in humans and is regarded as a global public health issue. The aim of this study is to identify agfA and mgtC genes in salmonella typhimurium strains isolated from stool samples using multiplex PCR method and to determine the resistance patterns of these strains.

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  • Primary care physician knowledge, attitudes, and ...

    2020-1-14 · Background Norovirus is a leading cause of acute gastroenteritis (AGE) across the age spectrum; candidate vaccines are in clinical trials. While norovirus diagnostic testing is increasingly available, stool testing may not be performed routinely, which can hamper surveillance and burden of disease estimates. Additionally, lack of …

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  • Oral Ondansetron for Gastroenteritis in a

    2014-9-5 · Lastly, an aptamer magnetic capture (AMC) method using aptamer 25 coupled with RT-qPCR was developed for recovery and detection of HuNoV in artificially contaminated lettuce. The capture efficiency of the AMC was 2.5-36% with an assay detection limit of 10 RNA copies per lettuce sample.

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  • Selection, Characterization and Application of

    2010-9-17 · 2005) and 0.79 pppy for ‘acute gastroenteritis’ in Americans of all ages (Mead et al. 1999) − i.e., in the developing-country range shown in the table. Source: Mathers et al. (2002). In low- and middle-income countries diarrhoeal diseases caused a total DALY loss of 59 million in 2001 (Lopez et al. 2006, Ezzati

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  • Discussion PaPer: oPtions for uPDatinG tHe 2006

    Abstract: Gastroenteritis and hepatitis, caused by human noroviruses (HuNoVs) and hepatitis A virus (HAV), respec-tively, are the most common illnesses resulting from the consumption of food contaminated with human enteric viruses. Food-grade polymers can be tailor designed to improve food safety, either as novel food-packaging materials …

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  • Viral gastroenteritis (stomach flu) - Symptoms

    2021-3-9 · Human noroviruses are one of the main causes of foodborne illnesses and represent a serious public health concern. Rapid and sensitive assays for human norovirus detection are undoubtedly necessary for clinical diagnosis, especially in regions without more sophisticated equipment. The rapid reverse transcription recombinase-aided amplification …

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  • Polymers and Biopolymers with Antiviral Activity ...

    2014-9-29 · Background Conventional reverse transcription-polymerase chain reaction (RT-PCR) amplification of the RNA-dependent RNA polymerase (RdRp) gene remains a used method for the rapid detection of norovirus (NV) in clinical laboratories. The incidence of and factors associated with false positives in this assay have not been …

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  • Appropriate management of acute

    2019-11-7 · Objectives To determine the proportion of care provided to children with acute gastroenteritis (AGE) in Australia consistent with clinical practice guidelines. Methods Indicators were developed from national and international clinical practice guideline (CPG) recommendations and validated by an expert panel. Medical records …

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  • Management of Acute Gastroenteritis in Children

    1999-12-1 · Acute gastroenteritis is a common and costly clinical problem in children. It is a largely self-limited disease with many etiologies. The evaluation of the child with acute gastroenteritis …

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  • Guidelines for managing acute gastroenteritis

    2012-9-23 · In this paper, we describe an affordable paramagnetic technology method using BioMag Carboxyl beads. Compared to other well-established, proprietary methods for purification of cycle sequencing products, this method produced consistently good results, with a very low reagent cost and short procedure time. 1.

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  • Cost-Effective Paramagnetic Bead Technique for ...

    Gastroenteritis is a short-term illness triggered by the infection and inflammation of the digestive system. Symptoms can include abdominal cramps, diarrhoea and vomiting. Some of the causes of gastroenteritis include viruses, bacteria, bacterial toxins, parasites, particular chemicals and some drugs. Good old-fashioned handwashing with soap ...

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  • Gastroenteritis - Better Health Channel

    2009-2-26 · Method: During upper endoscopic examinations, one investigator performed the procedure and the second investigator look at the endoscopic image. The degree of gastritis and duodenitis is graded. The results of the grading are blinded to the other investigator. The endoscopic video is stored for reassessment to determine the intra …

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  • Is a Reporting System for Gastritis or Duodenitis ...

    2018-12-12 · Psidium guajava (guava) is well known tropic tree which is abundantly grown for fruit. Many countries have a long history of using guava for medicinal purposes. This plant finds applications for the treatment of diarrhea, dysentery, gastroenteritis, hypertension, diabetes, caries and pain relief and for improvement in locomotors …

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  • How to Treat Gastroenteritis or Bowel

    2021-4-26 · The abscissa represents the feature importance calculated using the Gini index as an indicator. The ordinate is the name of the feature we considered in the model. Because there are many features that are used as model input, we add up features that account for a very small proportion and denote them as Other Feature.

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  • Continuous Enteral Rehydration by Nasogastric

    2020-9-18 · When oral rehydration is impossible, enteral rehydration via the nasogastric route has been the recommended method of rehydration since 2008 by ESPGHAN ( European Society for Paediatric Gastroenterology Hepatology and Nutrition ), for children with acute gastroenteritis. However, these recommendations are rarely followed in …

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  • 510(k) SUBSTANTIAL EQUIVALENCE

    2010-11-29 · colitis or gastroenteritis. The following pathogen types, subtypes and toxin genes are identified using the xTAG® GPP: · Campylobacter (C. jejuni, C. coli and C. lari only) · Clostridium difficile (C. difficile) toxin A/B · Cryptosporidium (C. parvum and C. hominis only) · Escherichia coli (E. coli) O157 · Enterotoxigenic

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  • An acute gastroenteritis outbreak associated

    2021-1-1 · Viruses associated with acute gastroenteritis were tested using real-time PCR. Capsid gene fragment of 557 bp of norovirus was amplified and sequenced. The phylogenetic tree was constructed with MEGA 7.0 using neighbor-joining method based on capsid gene fragment of norovirus. Results A total of 143 suspected cases were …

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  • A Study to Assess AK002 in Eosinophilic

    2020-3-26 · This is a Phase 3, multi-center, randomized, double-blind, placebo-controlled study to assess the efficacy and safety of lirentelimab (AK002), given monthly for 6 doses, in patients with moderately to severely active Eosinophilic Gastritis and/or Eosinophilic Duodenitis (formerly referred to as Eosinophilic Gastroenteritis) who have an ...

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  • Reduction in Rotavirus-associated Acute

    2020-12-23 · In Australia, prior to the introduction of vaccines, rotavirus-associated gastroenteritis was responsible for an estimated 10,000 hospital admissions annually, representing 50% of all gastroenteritis admissions. 1–3 Rotaviral disease has been estimated to cause 22,000 emergency department (ED) presentations and 115,000 …

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  • Differential Dx strategies - University of

    2017-11-14 · Practice this using a number of strategies with a few different complaints. See what you come up with. Here is a list of strategies with descriptions and examples of how they may be useful. Name of strategy What it is good for (examples) Comments • Symptom Complexes • Everything • A good database is needed. • Local anatomic …

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  • Multiplex Reverse Transcription-PCR for Rapid

    2006-5-1 · Jung, K, Chae, C: 2005, RT-PCR-based dot blot hybridization for the detection and differentiation between porcine epidemic diarrhea virus and transmissible gastroenteritis virus in fecal samples using a non-radioactive digoxigenin cDNA probe.

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  • Rotavirus Diagnosis - News-Medical.net

    2021-4-20 · Rotavirus represents the leading cause of severe childhood gastroenteritis. Every year rotavirus is thought to be responsible for approximately 25 million clinic visits, two million hospital ...

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  • ORIGINAL ARTICLE Antiviral efficacy and

    calicivirus, MS2 and ΦX-174 bacteriophages) using juices and extracts (not essential oils) from a single plant. Examples are studies with grape seed extract (Su and D’Souza 2011; Li et al. 2012), cranberry juice and cran-berry proanthocyanidins (Su et al. 2010a,b), and pome-granate juice and pomegranate polyphenols (Su et al. 2011).

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  • Salmonella nomenclature in the genomic era: a

    2021-4-5 · Using this method, 99.96% of Salmonella isolates reported in the UK were assigned a MAC type and linked to a serovar name …

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